Early Diagnosis of Hepatitis B by Dane Particle Associated DNA Polymerase Assay
نویسندگان
چکیده
منابع مشابه
Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction.
The circular DNA of hepatitis B Dane particles, which serves as the primer/template for an endogenous DNA polymerase, was analyzed by electrophoresis before and after a polymerase reaction and after digestion by restriction endonuclease or single-strand-specific endonuclease S1. The unreacted molecules extracted from the particles were electrophoretically heterogeneous, and treatment with S1 nu...
متن کاملDNA synthesized in the hepatitis B Dane particle DNA polymerase reaction.
Radioactive DNA was prepared in extensive (4 h) Dane particle DNA polymerase reactions. In different experiments the amount of new DNA, determined by the amount of nucleotide incorporation into an acid-insoluble form, was between 29 and 45% of the total circular DNA isolated from Dane particle preparations after the reaction. DNA reassociation kinetics were used to determine the complexity of t...
متن کاملDetection of DNA polymerase activities associated with purified duck hepatitis B virus core particles by using an activity gel assay.
Replication of hepadnaviruses involves reverse transcription of an intermediate RNA molecule. It is generally accepted that this replication scheme is carried out by a virally encoded, multifunctional polymerase which has DNA-dependent DNA polymerase, reverse transcriptase, and RNase H activities. Biochemical studies of the polymerase protein(s) have been limited by the inability to purify usef...
متن کاملRelease of e antigen from a Dane particle-rich preparation of hepatitis B virus.
Tween 80 treatment of a Dana particle-rich pellet obtained from sera of a carrier with a hepatitis type B infection resulted in the release of e antigen.
متن کاملMicromethod for phosphonoformate inhibition assay of hepatitis B viral DNA polymerase.
A micromethod for the specific measurement of hepatitis B viral DNA polymerase in serum is presented, based on the phosphonoformate inhibition assay (J Med Virol 12: 61-70, 1983). In the micromethod, sample volume is reduced to 120 microL and the ultracentrifugation step is eliminated. The method allows good discrimination between serum infected with hepatitis B virus and uninfected serum. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1977
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-36-1-217